Composite

Part:BBa_K4308031:Design

Designed by: Jun Zhang   Group: iGEM22_CPU_CHINA   (2022-10-08)


Cas12a1-crRNA-LINC00857


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 992
    Illegal BglII site found at 1325
    Illegal BglII site found at 1895
    Illegal BglII site found at 2162
    Illegal BglII site found at 2783
    Illegal BglII site found at 3759
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1054
    Illegal NgoMIV site found at 2053
    Illegal NgoMIV site found at 2755
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 427
    Illegal BsaI.rc site found at 1489
    Illegal BsaI.rc site found at 1669
    Illegal BsaI.rc site found at 2029
    Illegal SapI.rc site found at 1383
    Illegal SapI.rc site found at 1443
    Illegal SapI.rc site found at 2294
    Illegal SapI.rc site found at 2314
    Illegal SapI.rc site found at 2934


Design Notes

none


Source

none

References